| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Hearn, Ryleigh | D-West | 58.62 |
| 2 | Clark, Paige | Avon Grove | 1:01.00 |
| 3 | Veenema, Eden | Avon Grove | 1:01.17 |
| 4 | Conlan, Leia | Avon Grove | 1:01.50 |
| 5 | Keegan, Bridget | Avon Grove | 1:02.00 |
| 6 | Massey, Nia | D-East | 1:02.36 |
| 7 | Domurat, Chloe | Coatesville | 1:02.42 |
| 8 | Ciuffetelli, Eva | Avon Grove | 1:03.00 |
| 9 | Henning, Lily | D-West | 1:03.13 |
| 10 | Miller, Olivia | Bishop Shanahan | 1:03.43 |
| 11 | Marasco, Julia | D-West | 1:03.72 |
| 12 | Barcalow, Carlene | D-West | 1:04.10 |
| 13 | Driscoll, Delaney | Avon Grove | 1:04.50 |
| 14 | Ziemniak, Weronica | WC Henderson | 1:04.56 |
| 15 | Dever, Olivia | D-East | 1:04.72 |
| 16 | Young, Isis | D-East | 1:04.94 |
| 17 | Reed, Lauren | Coatesville | 1:04.95 |
| 18 | Kyle, Nora | Bishop Shanahan | 1:05.14 |
| 19 | Chylack, Carlie | D-West | 1:05.21 |
| 20 | Blanchette, Emma | D-West | 1:06.72 |
| 21 | Witkowski, Ava | D-West | 1:06.81 |
| 22 | Uhlman, Deborah | Bishop Shanahan | 1:07.12 |
| 23 | Schindler, Lily | Avon Grove | 1:07.47 |
| 24 | Cooper, Jada | WC East | 1:07.50 |
| 25 | Shearer, Cecilia | D-West | 1:08.30 |
| 26 | Figueroa, Adrianna | Coatesville | 1:08.36 |
| 27 | Keefe, Maeve | WC East | 1:08.66 |
| 28 | Brown, Payton | Avon Grove | 1:09.60 |
| 29 | Michell, Molly | WC Henderson | 1:09.89 |
| 30 | Fielding, Hannah | WC Henderson | 1:10.18 |
| 31 | Moore, Keegan | D-East | 1:10.24 |
| 32 | Schottin, Bailey | Avon Grove | 1:10.48 |
| 33 | Whelan, Morgan | D-West | 1:10.66 |
| 34 | Caprarola, Reagan | Avon Grove | 1:11.80 |
| 35 | Kleckner, Zoe | WC Henderson | 1:12.11 |
| 36 | Mineweaser, Brooklyn | Bishop Shanahan | 1:12.62 |
| 37 | Klara, Rachel | WC Henderson | 1:12.91 |
| 38 | Williams, Rose | Bishop Shanahan | 1:13.36 |
| 39 | Bradshaw, Genesis | Coatesville | 1:13.45 |
| 40 | McGonagle, Meghan | Bishop Shanahan | 1:13.82 |
| 41 | Nolan, Zoe | WC Henderson | 1:13.87 |
| 42 | Rosado, Ciana | Coatesville | 1:13.90 |
| 43 | Hangey, Amelie | Bishop Shanahan | 1:14.27 |
| 44 | Bachman, Claire | WC Henderson | 1:14.28 |
| 45 | Durell, Lailah | Coatesville | 1:14.69 |
| 46 | Eleby, Paris | Coatesville | 1:15.00 |
| 47 | Heraghty, Niamh | WC Henderson | 1:15.08 |
| 48 | Coghan, Caroline | Bishop Shanahan | 1:15.40 |
| 49 | Skomorucha, Leah | WC Henderson | 1:15.93 |
| 50 | Friz, Grace | Bishop Shanahan | 1:16.00 |
| 51 | Le, Dora | Bishop Shanahan | 1:16.01 |
| 52 | Cohen, Maddox | WC Henderson | 1:16.24 |
| 53 | Young, Abby | D-West | 1:16.49 |
| 54 | Richardson, Kerry | WC East | 1:17.72 |
| 55 | Fernandes, Kasey | WC East | 1:21.68 |