| # | Athlete | Yr. | Team | Seed | |
|---|---|---|---|---|---|
| 1 | 683 | Silvester, Tommy | SR | Coatesville | 49.19 |
| 2 | 685 | Stocker, Jabarri | SR | Coatesville | 49.49 |
| 3 | 901 | Kolade, Olo | SO | Downingtown West | 49.78 |
| 4 | 906 | Wade, Josiah | SO | Downingtown West | 50.12 |
| 5 | 601 | Polizzi, Jack | JR | Bishop Shanahan | 50.40 |
| 6 | 679 | Dimatteo, Phillip | SO | Coatesville | 51.14 |
| 7 | 1052 | Balick, Samuel | SR | Avon Grove | 51.30 |
| 8 | 804 | Baum, Alexander | SR | West Chester Henderson | 51.36 |
| 9 | 785 | Byrnes, Gavin | SR | West Chester Henderson | 51.48 |
| 10 | 598 | Okafor, Chijioke | SR | Bishop Shanahan | 51.84 |
| 11 | 988 | Kellogg, Brad | SR | Downingtown East | 51.90 |
| 12 | 900 | Casner, Zaire | SR | Downingtown West | 52.00 |
| 13 | 681 | Morris, Elijah | JR | Coatesville | 52.18 |
| 14 | 903 | Planck, Richard | SR | Downingtown West | 52.24 |
| 15 | 792 | Walker, Christian | SR | West Chester Henderson | 52.29 |
| 16 | 902 | Leisenring, Sam | JR | Downingtown West | 52.35 |
| 17 | 739 | McCaffrey, Stephen | SR | West Chester East | 52.44 |
| 18 | 805 | Filipas, Michael | SR | West Chester Henderson | 52.46 |
| 19 | 888 | Sterling, Amani | JR | Downingtown West | 53.00 |
| 20 | 989 | Richards, Niall | JR | Downingtown East | 53.05 |
| 21 | 687 | Wilson, Jelani | SR | Coatesville | 53.40 |
| 22 | 992 | Taylor, Liam | SR | Downingtown East | 53.45 |
| 23 | 600 | Palaia, Greg | SO | Bishop Shanahan | 53.62 |
| 24 | 984 | Beck, Caleb | SR | Downingtown East | 54.24 |
| 25 | 904 | Showers, Brayden | SR | Downingtown West | 54.84 |
| 26 | 1074 | Pendse, Nikhil | SO | Avon Grove | 54.84 |
| 27 | 603 | Todd, Steven | JR | Bishop Shanahan | 54.97 |
| 28 | 905 | Ulshafer, Ethan | SR | Downingtown West | 55.15 |
| 29 | 809 | Zurn, Mason | 9 | West Chester Henderson | 55.25 |
| 30 | 985 | Beck, Christian | SO | Downingtown East | 55.39 |
| 31 | 738 | Gallagher, Aidan | SR | West Chester East | 55.44 |
| 32 | 899 | Benner, Dylan | SR | Downingtown West | 55.45 |
| 33 | 808 | Varghese, Aaron | SR | West Chester Henderson | 55.45 |
| 34 | 579 | Conte, Samuel | JR | Bishop Shanahan | 55.46 |
| 35 | 990 | Robson, Tommy | SO | Downingtown East | 55.65 |
| 36 | 806 | Hogan, Dermot | SO | West Chester Henderson | 55.66 |
| 37 | 1076 | Trone, Lars | JR | Avon Grove | 56.00 |
| 38 | 604 | Whitaker, Owen | JR | Bishop Shanahan | 56.10 |
| 39 | 790 | Soler, Aidan | SO | West Chester Henderson | 56.20 |
| 40 | 983 | Bablani, Aryav | 9 | Downingtown East | 56.24 |
| 41 | 680 | Green, Cameron | SO | Coatesville | 56.56 |
| 42 | 807 | Smith, Connor | 9 | West Chester Henderson | 56.95 |
| 43 | 724 | Olano-Gil, Dylan | 9 | West Chester East | 57.08 |
| 44 | 791 | Subratie, Aniff | JR | West Chester Henderson | 57.50 |
| 45 | 682 | Ochola, Camayo | JR | Coatesville | 57.55 |
| 46 | 684 | Soto-Villafane, Abrian | SR | Coatesville | 57.70 |
| 47 | 1072 | Kochhar, Krish | JR | Avon Grove | 58.00 |
| 48 | 686 | Wagner, Ryan | SO | Coatesville | 58.08 |
| 49 | 597 | Kyle, James | 9 | Bishop Shanahan | 58.23 |
| 50 | 987 | Frantz, Brady | 9 | Downingtown East | 58.24 |
| 51 | 599 | Okafor, Osinachi | SO | Bishop Shanahan | 58.24 |
| 52 | 1075 | Shah, Trevor | JR | Avon Grove | 58.73 |
| 53 | 740 | Rivera, Luis | SO | West Chester East | 58.94 |
| 54 | 1071 | Adams, Dylan | SO | Avon Grove | 59.00 |
| 55 | 1073 | MacGregor, William | SO | Avon Grove | 1:00.00 |
| 56 | 986 | Blevins, Justin | 9 | Downingtown East | 1:00.24 |
| 57 | 991 | Rohrbaugh, Jack | 9 | Downingtown East | 1:00.24 |
| 58 | 602 | Styer, Samuel | SO | Bishop Shanahan | 1:02.40 |
| 59 | 678 | De Shields, A' mari | SO | Coatesville | 1:02.40 |
| 60 | 737 | Crooms, Prince | JR | West Chester East | 1:02.51 |
| 61 | 741 | Varkey, Jayden | JR | West Chester East | 1:02.76 |
| 62 | 578 | Conklin, William | SO | Bishop Shanahan | 1:04.96 |