| Lane | Athlete | Team | Seed | 
|---|---|---|---|
| 1 | Caronia, Alex | WC Henderson | 55.79 | 
| 2 | Compher, Emma | D-West | 52.16 | 
| 3 | Ericson, Rylee | D-East | 50.37 | 
| 4 | Smith, Payton | Avon Grove | 48.52 | 
| 5 | Eberly, Lorelei | Coatesville | 49.20 | 
| 6 | Hearn, Ryleigh | D-West | 51.50 | 
| 7 | Baumann, Chloe | D-East | 54.55 | 
| 8 | Jennings-Scott, Giovanna | D-East | 56.24 | 
| Lane | Athlete | Team | Seed | 
|---|---|---|---|
| 1 | van der Steur, Anastasia | Bishop Shanahan | 58.61 | 
| 2 | Uhlman, Deborah | Bishop Shanahan | 58.37 | 
| 3 | Shaefer, Reilly | D-East | 57.36 | 
| 4 | Brunschwyler, Charlotte | D-West | 57.00 | 
| 5 | Hauer, Carly | D-East | 57.28 | 
| 6 | Nolan, Zoe | WC Henderson | 58.04 | 
| 7 | Yarnall-Stevenson, Charlotte | D-East | 58.57 | 
| 8 | Montgomery, Alexis | D-West | 58.79 | 
| Lane | Athlete | Team | Seed | 
|---|---|---|---|
| 1 | Drucis, Anna | WC East | 1:03.00 | 
| 2 | Keefe, Maeve | WC East | 1:01.00 | 
| 3 | Sujesh, Keerthana | D-East | 59.57 | 
| 4 | Hangey, Rebecca | Bishop Shanahan | 59.00 | 
| 5 | Fuhr, Mya | D-West | 59.22 | 
| 6 | Davies, Grace | D-East | 1:00.82 | 
| 7 | Carlow, Ana | WC Henderson | 1:02.20 | 
| 8 | Robinson, Julia | D-East | 1:03.76 | 
| Lane | Athlete | Team | Seed | 
|---|---|---|---|
| 3 | Rogelstad, Sarah | Bishop Shanahan | NT | 
| 4 | Bousquet, Sophie | D-East | 1:08.68 | 
| 5 | Conrad, Caroline | WC Henderson | 1:17.07 |