| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Piombino, Sonia | WC East | 2:21.24 |
| 2 | Mulhern, Jenna | WC Henderson | 2:22.62 |
| 3 | Henry, Paige | Coatesville | 2:25.93 |
| 4 | Willis, Meghan | Bishop Shanahan | 2:26.00 |
| 5 | Ziemniak, Weronica | WC Henderson | 2:27.17 |
| 6 | Kiey, Bridget | D-West | 2:27.19 |
| 7 | Brown, Madison | D-East | 2:27.26 |
| 8 | Okumura, Gabrielle | Coatesville | 2:28.90 |
| 9 | Kyle, Nora | Bishop Shanahan | 2:29.00 |
| 10 | Schiele, Michaela | WC East | 2:30.24 |
| 11 | Allison, Maeve | Avon Grove | 2:32.55 |
| 12 | Pfaff, Ava | Avon Grove | 2:33.00 |
| 13 | Witkowski, Ava | D-West | 2:33.15 |
| 14 | Diebold, Oriah | D-West | 2:33.24 |
| 15 | Keegan, Maggie | Avon Grove | 2:33.32 |
| 16 | Hufnagel, Aubrey | D-West | 2:34.35 |
| 17 | Buffington, Fiona | Avon Grove | 2:35.00 |
| 18 | Oskison, Paige | D-West | 2:35.24 |
| 19 | McGinty, Hannah | D-East | 2:36.13 |
| 20 | Greulich, Marguerite | WC East | 2:36.98 |
| 21 | DiEgidio, Ava | WC East | 2:37.60 |
| 22 | Howe, Bridget | D-West | 2:37.63 |
| 23 | Shaw, Savannah | D-West | 2:37.95 |
| 24 | Ward, Jessica | Bishop Shanahan | 2:39.00 |
| 25 | Barfell, Brooke | Avon Grove | 2:39.11 |
| 26 | McErlean, Arden | D-West | 2:40.87 |
| 27 | Caronia, Gabriella | WC Henderson | 2:41.00 |
| 28 | Fielding, Hannah | WC Henderson | 2:41.10 |
| 29 | Chandler, Addison | Avon Grove | 2:42.41 |
| 30 | Andiario, Rachel | WC East | 2:44.66 |
| 31 | Hewitt, Evelyn | Bishop Shanahan | 2:45.00 |
| 32 | Ward, Kirsten | Bishop Shanahan | 2:46.00 |
| 33 | Omiridis, Marissa | WC Henderson | 2:46.67 |
| 34 | Halladay, Jane | WC Henderson | 2:48.20 |
| 35 | Duffy, Elise | D-West | 2:48.71 |
| 36 | Chermak, Carly | WC East | 2:49.24 |
| 37 | McCaffrey, Lily | WC East | 2:49.24 |
| 38 | Cipriano, Isabelle | Bishop Shanahan | 2:50.00 |
| 39 | Tsantrizos, Helena | D-East | 2:52.24 |
| 40 | O'Brian, Grace | Coatesville | 2:54.00 |
| 41 | Kleckner, Emma | WC Henderson | 2:54.30 |
| 42 | Kriebel, Ava | WC Henderson | 2:55.70 |
| 43 | Littlefield, Kala | D-West | 2:55.95 |
| 44 | LaVeyra, Adylee | D-East | 2:57.24 |
| 45 | Klein, Noelle | Bishop Shanahan | 2:59.00 |
| 46 | Dinh, Thanh-Lan | D-East | 2:59.24 |
| 47 | Nazareth, Maryann | D-East | 2:59.77 |
| 48 | Lacey, Jenna | D-East | 3:01.24 |
| 49 | Cuesta, Julia | WC Henderson | 3:02.26 |
| 50 | Chan, Abbey | Bishop Shanahan | 3:03.00 |
| 51 | Olivo, Madeline | Bishop Shanahan | NT |
| 52 | Williams, Rose | Bishop Shanahan | NT |