| # | Athlete | Yr. | Team | Seed | |
|---|---|---|---|---|---|
| 1 | 845 | Weitzman, Molly | JR | Downingtown West | 56.66 |
| 2 | 952 | Schindler, Lily | SR | Avon Grove | 57.74 |
| 3 | 385 | Nolan, Payton | SO | Bishop Shanahan | 59.57 |
| 4 | 865 | Christman, Ja'lyncia | SO | Coatesville | 59.68 |
| 5 | 862 | Weldon, Ava | SO | Coatesville | 1:00.01 |
| 6 | 910 | Ziemniak, Julia | JR | West Chester Henderson | 1:00.31 |
| 7 | 55 | Daversa, Katrina | JR | Downingtown East | 1:00.63 |
| 8 | 59 | Dever, Olivia | SR | Downingtown East | 1:01.07 |
| 9 | 818 | Miller, Gabriella | JR | Downingtown West | 1:01.24 |
| 10 | 241 | DeAngelis, Sophia | FR | Downingtown West | 1:01.37 |
| 11 | 116 | Zajac, Julie | JR | Downingtown East | 1:01.77 |
| 12 | 852 | Wilford, Simone | FR | Downingtown West | 1:02.00 |
| 13 | 944 | Keegan, Bridget | SR | Avon Grove | 1:02.00 |
| 14 | 189 | Milbourne, Samiya | 8 | Downingtown West | 1:03.00 |
| 15 | 829 | Bayley, Morgan | JR | Downingtown West | 1:03.24 |
| 16 | 810 | Mazzone, Giuliana | SO | Downingtown West | 1:03.24 |
| 17 | 807 | Coyle, Maia | JR | Downingtown West | 1:03.24 |
| 18 | 877 | Bradshaw, Genesis | SR | Coatesville | 1:03.40 |
| 19 | 864 | Boyer, Amauri | JR | Coatesville | 1:03.87 |
| 20 | 555 | Miller, Kennedy | SO | West Chester Henderson | 1:04.03 |
| 21 | 926 | Rieger, Brynlea | JR | West Chester Henderson | 1:04.08 |
| 22 | 27 | Miller, Hannah | JR | Downingtown East | 1:04.13 |
| 23 | 525 | Miller, Kaitlyn | JR | West Chester Henderson | 1:04.27 |
| 24 | 294 | Murungi, Jasmin | SO | Coatesville | 1:04.93 |
| 25 | 951 | Sullivan, Emily | SO | Avon Grove | 1:05.00 |
| 26 | 936 | Rowe, Lyla | JR | Avon Grove | 1:05.00 |
| 27 | 947 | Hopkins, Chloe | SO | Avon Grove | 1:05.00 |
| 28 | 414 | Kruzek, Madeline | FR | Bishop Shanahan | 1:05.45 |
| 29 | 895 | Cooper, Jada | SR | West Chester East | 1:05.68 |
| 30 | 126 | Main, Avery | FR | Downingtown East | 1:05.72 |
| 31 | 118 | Kinne, Lindsey | SO | Downingtown East | 1:05.94 |
| 32 | 750 | Lipstas, Angelina | FR | Avon Grove | 1:06.00 |
| 33 | 321 | Fowkles, Kaylee | JR | Coatesville | 1:06.20 |
| 34 | 330 | Hadley, Aden | JR | Coatesville | 1:06.26 |
| 35 | 448 | Bechtel, Claire | FR | West Chester East | 1:06.47 |
| 36 | 634 | Malizia, Marlee | FR | West Chester Henderson | 1:06.52 |
| 37 | 927 | Sellers, Annie | JR | West Chester Henderson | 1:06.60 |
| 38 | 665 | Dirani, Anna | JR | West Chester Henderson | 1:06.73 |
| 39 | 730 | Timm, Natalie | SO | Avon Grove | 1:06.77 |
| 40 | 573 | Plavcan, Collette | JR | West Chester Henderson | 1:06.82 |
| 41 | 832 | Brochet, Lizzie | JR | Downingtown West | 1:06.84 |
| 42 | 594 | Smith, Madison | FR | West Chester Henderson | 1:06.86 |
| 43 | 409 | Longenderfer, Madison | JR | Bishop Shanahan | 1:06.94 |
| 44 | 80 | Sebby, Molly | SO | Downingtown East | 1:06.95 |
| 45 | 893 | Elgedawy, Mentalla | JR | West Chester East | 1:07.26 |
| 46 | 371 | Regan, Josephine | JR | Bishop Shanahan | 1:08.20 |
| 47 | 310 | Dixon, Jayden | SO | Coatesville | 1:08.50 |
| 48 | 714 | Roberts, Yiana | JR | Avon Grove | 1:09.80 |
| 49 | 238 | Riddick, Jasmine | FR | Downingtown West | 1:09.87 |
| 50 | 689 | Taylor, Adalynn | SO | Avon Grove | 1:09.91 |
| 51 | 380 | Ashman, Ciall | JR | Bishop Shanahan | 1:10.00 |
| 52 | 455 | Diasseny, Sharifa | JR | West Chester East | 1:10.00 |
| 53 | 326 | Alston, Bryzhae | SO | Coatesville | 1:10.10 |
| 54 | 305 | Carter, Sanai | JR | Coatesville | 1:10.33 |
| 55 | 606 | Olsen, Jane | SO | West Chester Henderson | 1:10.43 |
| 56 | 900 | Makos, Savanna | SO | West Chester East | 1:11.03 |
| 57 | 348 | Bonass, Fiona | FR | Bishop Shanahan | 1:11.23 |
| 58 | 520 | Dwinnells, Ashleee | FR | West Chester East | 1:11.24 |
| 59 | 778 | Miller, Natalie | JR | Avon Grove | 1:11.33 |
| 60 | 467 | Ziegler, Calleigh | West Chester East | 1:12.17 | |
| 61 | 412 | Hart, Megan | FR | Bishop Shanahan | 1:12.61 |
| 62 | 441 | Brittingham, Caroline | JR | West Chester East | 1:12.86 |
| 63 | 381 | Jascewsky, Juliana | JR | Bishop Shanahan | 1:13.81 |
| 64 | 495 | Loveland, Payton | FR | West Chester East | 1:14.35 |
| 65 | 360 | Schmid, Jessica | FR | Bishop Shanahan | 1:15.00 |
| 66 | 384 | Logue, Shannon | FR | Bishop Shanahan | 1:16.28 |
| 67 | 965 | McElhiney, Peyton | West Chester Henderson | NT |