| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Sujesh, Keerthana | Downingtown East | 50.05 |
| 2 | Compher, Emma | Downingtown West | 51.11 |
| 3 | Fuhr, Mya | Downingtown West | 51.78 |
| 4 | Hangey, Rebecca | Bishop Shanahan | 52.24 |
| 5 | Uhlman, Deborah | Bishop Shanahan | 53.62 |
| 6 | Yarnall-Stevenson, Charlotte | Downingtown East | 54.71 |
| 7 | Nolan, Zoe | West Chester Henderson | 55.26 |
| 8 | Davies, Grace | Downingtown East | 55.36 |
| 9 | Manuel, Kaila | Downingtown East | 55.52 |
| 10 | Schaefer, Reilly | Downingtown East | 56.11 |
| 11 | Guru, Bhavya | Downingtown East | 56.55 |
| 12 | Loeper, Penelope | Bishop Shanahan | 56.78 |
| 13 | Finkbiner, Ashley | Downingtown West | 57.40 |
| 14 | Brazunas, Mckenna | Downingtown East | 57.44 |
| 15 | Rogelstad, Sarah | Downingtown West | 57.73 |
| 16 | Klara, Olivia | West Chester Henderson | 58.14 |
| 17 | Skomorucha, Leah | West Chester Henderson | 58.16 |
| 18 | Gray, Leah | Avon Grove | 1:00.18 |
| 19 | Blake, Meghan | Downingtown East | 1:01.65 |
| 20 | Robinson, Julia | Downingtown East | 1:02.61 |
| 21 | Lueders, Mackenzie | Downingtown East | 1:02.67 |
| 22 | Hand, Julia | Downingtown East | 1:03.81 |
| 23 | Cao, Xinnuo | Downingtown East | 1:04.15 |
| 24 | Conrad, Caroline | West Chester Henderson | 1:05.49 |
| 25 | Senthikumar, Sagarika | Downingtown East | NT |
| 26 | Friz, Grace | Bishop Shanahan | NT |
| 27 | deans, amaya | Downingtown East | NT |
| 28 | Xu, Melody | Downingtown East | NT |
| 29 | Baumeister, Jess | Downingtown East | NT |