| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | Keegan, Bridget | Avon Grove | 49.00 |
| 2 | Sujesh, Keerthana | Downingtown East | 49.13 |
| 3 | Ziemniak, Julia | West Chester Henderson | 53.50 |
| 4 | Jennings-Scott, Giovanna | Downingtown East | 54.03 |
| 5 | Yarnall-Stevenson, Charlotte | Downingtown East | 54.49 |
| 6 | Manuel, Kaila | Downingtown East | 55.52 |
| 7 | Finkbiner, Ashley | Downingtown West | 55.58 |
| 8 | Veenema, Mia | Avon Grove | 56.00 |
| 9 | Hand, Julia | Downingtown East | 56.01 |
| 10 | Guru, Bhavya | Downingtown East | 56.55 |
| 11 | McLean, Elissa | Avon Grove | 57.00 |
| 12 | Soto-Villafane, Jaylin | Coatesville | 57.26 |
| 13 | Senthikumar, Sagarika | Downingtown East | 57.61 |
| 14 | Tras, Ruhi | West Chester Henderson | 58.00 |
| 15 | Hnat, Olivia | West Chester Henderson | 58.00 |
| 16 | Lueders, Mackenzie | Downingtown East | 58.74 |
| 17 | Gray, Leah | Avon Grove | 59.07 |
| 18 | Botchway, Makena | Coatesville | 59.90 |
| 19 | Robinson, Julia | Downingtown East | 1:01.83 |
| 20 | Olson, Harmony | Downingtown West | 1:05.00 |
| 21 | Ashman, Ciall | Bishop Shanahan | NT |
| 22 | Colmenares-Bittar, Corina | Downingtown East | NT |
| 23 | Kyada, Shaili | Downingtown East | NT |