| Lane | # | Athlete | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | 252 | FOSTER, Jade | West Charlotte | 2:30.00 | Unverified: |
| 2 | 442 | MOYER, Claire | East Mecklenburg | 2:34.98 | Verified |
| 3 | 817 | EDWARDS, Phoebe | Butler | 2:36.69 | Verified |
| 4 | 444 | PALMER, Megan | East Mecklenburg | 2:40.75 | Verified |
| 5 | 243 | DANCY, Mariah | West Charlotte | 2:48.00 | Unverified: |
| 6 | 820 | RIZO, Julimar | Butler | 2:50.20 | Verified |
| 7 | 743 | WALTON, Bhailei | Independence | 2:50.62 | Verified |
| 8 | 74 | DEAN, Piper | Hopewell | 2:51.80 | Unverified: Rocky River HS Meet #1 |
| 9 | 468 | BRIDGES, Mariah | East Mecklenburg | 2:52.14 | Verified |
| 10 | 68 | HEGE, Gabrielle | Hopewell | 2:52.40 | Verified |
| 11 | 448 | MOORE, Kynna | East Mecklenburg | 2:53.98 | Verified |
| 12 | 465 | CHANEY, Tabitha | East Mecklenburg | 3:02.60 | Verified |
| 13 | 291 | UKIO, Marian | West Charlotte | 3:50.22 | Verified |
| 14 | 783 | MOHAMMED, Rando | Independence | NT | Unverified: |
| 15 | 117 | GOMEZ ALONSO, Laia | Hopewell | NT | Unverified: |
| 16 | 874 | PATEL, Priana | Butler | NT | Unverified: |
| 17 | 763 | GEBREHIWOT, Leora | Independence | NT | Unverified: |
| 18 | 304 | CROSS, Milan | West Charlotte | NT | Unverified: |
| 19 | 760 | MCCORD, Iyana | Independence | NT | Unverified: |
| 20 | 863 | PRATT, Iris | Butler | NT | Unverified: |
| 21 | 818 | PATEL, Priana | Butler | NT |