| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | East Mecklenburg | B | Nyanforh | Williams | Chandler | Lindsey | 1:35.62 | Verified |
| 4 | Independence | A | Grant | Lofton | Crommarty | Massey | NT | Unverified: |
| 5 | East Mecklenburg | A | Daley | Grier | Alvin | Ausman | 1:40.62 | Unverified: Spring into the Season Kickoff |
| 6 | West Charlotte | A | Abner | Hudson | Manisak-Brownlee | Hudson | NT | Unverified: |