| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Garinger | A | NT | Verified | ||||
| 2 | East Mecklenburg | A | Bailey | Raysor | Kalgren | Adenuga | 4:14.46 | Verified |
| 3 | Butler | A | Butler | Collard | Ellis | Marshall | 4:12.61 | Verified |
| 4 | Independence | A | NT | Verified | ||||
| 5 | Providence | A | Weinke | Schubert | Caldwell | Hall | 4:25.82 | Verified |
| 6 | Charlotte Catholic | A | Bello | Richard | McArdle | Canon | 4:41.73 | Verified |