| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Independence | A | Davis | Garnes | Mulkey | Ben-Youssef | NT | Verified |
| 2 | Hopewell | A | Buriss | Kenley | Smith | Zamora | 3:38.93 | Verified |
| 3 | Providence | A | Brown | Monahan | Perrone | MAXWELL | NT | Verified |
| 4 | Atkins | A | Niang | Duke-Graves | Puryear | Allen | NT | Verified |
| 5 | West Charlotte | A | Moore | Whitlock | Gonzales | Hudson | NT | Verified |
| 6 | Julius Chambers High School | A | Williams | Morrow | Brooks | Smith | 3:56.11 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Palisades High School | A | Odom | Morgan | Garner-Townsend | Quarles | NT | Verified |
| 2 | Butler | A | NT | Verified | ||||
| 3 | Hough, William A | A | NT | Verified | ||||
| 4 | East Mecklenburg | A | Mason | Smith | Owens | Massey | 3:21.57 | Verified |
| 5 | Olympic | A | Snow | Wiley | Barnes | Ford | NT | Verified |
| 6 | Berry, Phillip O. | A | Davis | Mills | Britt | Bell | NT | Verified |