| Lane | # | Athlete | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | 210 | RAINEY, Reagan | Hough, William A | 5:03.79 | Verified |
| 2 | 529 | CHEN, Helen | Providence | 5:17.88 | Verified |
| 3 | 192 | BEYERLE, Jenna | Hough, William A | 5:24.57 | Verified |
| 4 | 536 | LYON, Rachel | Providence | 5:30.60 | Verified |
| 5 | 79 | EDWARDS, Phoebe | Butler | 5:36.77 | Verified |
| 6 | 170 | WIGGINTON, Kasey | Hopewell | 5:42.97 | Verified |
| 7 | 538 | MOORE, Jasmine | Providence | 5:43.63 | Verified |
| 8 | 415 | KOPPANG, Langston | Myers Park | 5:45.79 | Verified |
| 9 | 114 | DAVIS, Stewart | East Mecklenburg | 5:46.93 | Verified |
| 10 | 324 | WILLIAMS, MC | Lake Norman Charter School | 5:50.49 | Verified |
| 11 | 421 | SNOWDON, Ava | Myers Park | 5:51.30 | Verified |
| 12 | 410 | FLEURY, Amelia | Myers Park | 5:51.85 | Verified |
| 13 | 118 | MOYER, Claire | East Mecklenburg | 5:55.81 | Verified |
| 14 | 212 | RAMARAJU, Sonya | Hough, William A | 6:10.86 | Verified |
| 15 | 35 | BRINSON, Amariya | Berry, Phillip O. | 6:53.92 | Verified |
| 16 | 271 | CAUDLE, Amina | Julius Chambers High School | NT | Unverified: |
| 17 | 117 | KOLODZIEY, Hadley | East Mecklenburg | NT |