| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Butler | A | 52.20 | ||||
| 2 | Independence | A | Cabaniss-Ali | Dickerson | Fatu | Thompson | 54.78 |
| 3 | Myers Park | A | Trang | Mcallister | ELLIS | Mobley | 52.44 |
| 4 | North Mecklenburg | A | Daniels | Grier | Baxter | Michael | 54.64 |
| 5 | Hickory Grove Baptist Christian | A | Clark | Jones | Quarles | Smith | 59.47 |
| 6 | Julius Chambers High School | A | Goodwin | Johnson | Henderson | Strickland | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Hopewell | A | Terry-London | Coley | Gilcrest | Gray | 51.78 |
| 2 | Rocky River High School | A | Collins | Gordon | Sumo | Goode | DQ |
| 3 | Mallard Creek | A | Cherry | Woodward | Kamba | Steele | 47.55 |
| 4 | Olympic | A | Outen | Williamson | Wheeler | Jones | 49.22 |
| 5 | Cox Mill | A | Cherry | Koonce | Willams | Resse | 51.70 |