| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | St. Francis Borgia | A | Garcilaso, Shekinah | Comage, Bernadette | Glastetter, Martha | Pfeiffer, Diana | 1:54.00 |
| 4 | Francis Howell Central | A | Anderson, Tyla | Cooper, Rebecca | Ebert, Lauren | Hanna, Shante | 1:52.70 |
| 5 | Troy-Buchanan | A | Morice, Amanda | Beck, Haley | Matech-Edwards, Salena | Sullivan, Anna | 1:53.20 |
| 6 | St. Francis Borgia | B | Brinker, Samantha | Charboneau, Macey | Rembecki, Ana | Williams, Allison | 2:03.00 |