| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BATES, Alex | SO | Southwestern CC | 1:00.50 | 2022 Region XI NJCAA Championships |
| 2 | WOOLBRIGHT, Camden | JR | Missouri Baptist | 58.00 | |
| 3 | BRYANT, Ethan | FR | Saint Louis | 58.00 | |
| 4 | BAKER, James | JR | Saint Louis | 56.74 | 56.74 (400m IH 56.74) 2022 Kentucky Track & Field Invitational |
| 5 | GRAHAM, Keon | JR | Missouri Baptist | 57.87 | Miner Invitational |
| 6 | VICKREY, Chase | FR | Unattached | 58.00 | Billiken Invitational |
| 7 | DONOHUE, Nicoli | FR | Saint Louis | 59.00 | |
| 8 | GODSEY, Gage | FR | Southwestern CC | 1:01.35 | Grand View Viking Relays |