| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Health Sciences and Pharmacy | A | McPike | Thomas | Wakeland | Wallace | 4:30.27 | American Midwest Outdoor Track & Field Championships |
| 3 | Purdue Northwest | A | Testa | Jones | Bartlett | Schultze | 4:01.46 | https://tfrrs.org/results/75375/4629899/GVSU_Holiday_Open/4-x-400-Relay |
| 4 | Saint Louis | A | Jackson | O'Neil | Williams | Kieffer | 3:58.42 | 3:58.42 2023 Indiana University Relays |
| 5 | Saint Louis | B | McDowell | Murray | Rust | Doncevic | 4:00.00 | |
| 6 | Saint Louis | C | Morin | Monson | Wolf | Roelofse | 4:20.00 |