| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | ROBINSON, Alicia | FR | Harris-Stowe St. | 1:21.79 | Miner Invitational |
| 4 | FARCHMIN, Emily | Unattached | 30.00 | ||
| 5 | HOLM, Kyla | FR | Southwestern CC | 31.48 | NJCAA Region 11 Championship |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LONG, Shamari | SR | Harris-Stowe St. | 28.50 | Titan Open 2023 |
| 2 | BUDUAN, Arianna | FR | Health Sciences and Pharmacy | 28.33 | |
| 3 | HOGAN, Perri | JR | Missouri Baptist | 28.23 | Frigid Bee Invitational |
| 4 | LAWRENCE, Allie | FR | Health Sciences and Pharmacy | 27.34 | |
| 5 | MASSIER, Grace | SO | Southwestern CC | 27.97 | Dick Young Invitational |
| 6 | HUFFSTETTER, Madelyn | JR | Fontbonne | 28.33 | Titan Open 2023 |
| 7 | DELARUE, Gabby | JR | Missouri Baptist | 28.40 | |
| 8 | SALEH, Maegan | SR | Missouri Baptist | 28.62 | Frigid Bee Invitational |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILLIAMS, Zora | FR | Saint Louis | 26.56 | |
| 2 | ANDORF, Maddie | SR | Saint Louis | 26.06 | 26.06 2022 Hoosier Open |
| 3 | ROBINSON, Jaila | SR | Saint Louis | 25.74 | 25.74# (26.14) 2023 EIU Friday Night Special |
| 4 | NICHOLS, Emily | JR | Saint Louis | 25.39 | 2023 Boston University David Hemery Valentine Invite |
| 5 | MCDOWELL, Mikayla | SR | Saint Louis | 25.43 | 25.43 2022 Kentucky Track & Field Invitational |
| 6 | O'NEIL, Patricia | FR | Saint Louis | 26.00 | |
| 7 | JACKSON, Jillian | FR | Saint Louis | 26.49 | 2023 Blue Demon Alumni Classic |
| 8 | DONCEVIC, Dani | SO | Saint Louis | 26.97 | 2022 Kentucky Track & Field Invitational |