| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | HOLM, Kyla | FR | Southwestern CC | 14.50 | |
| 3 | HOGAN, Perri | JR | Missouri Baptist | 13.52 | Miner Invitational |
| 4 | MASSIER, Grace | SO | Southwestern CC | 13.35 | Central College Dutch Invite |
| 5 | BOSTIC, Grace | SO | Unattached | 13.50 | |
| 6 | LONG, Shamari | SR | Harris-Stowe St. | 13.64 | Clark Atlanta University Panther Invitational |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROBINSON, Alicia | FR | Harris-Stowe St. | 13.17 | Miner Invitational |
| 2 | SALEH, Maegan | SR | Missouri Baptist | 12.90 | AMC Conference Championships |
| 3 | ROBINSON, Jaila | SR | Saint Louis | 12.53 | Atlantic 10 Outdoor Championships |
| 4 | PHIPPS, A'Lexus | SR | Harris-Stowe St. | 12.10 | |
| 5 | ANDORF, Maddie | SR | Saint Louis | 12.33 | Billiken Invitational |
| 6 | HUFFSTETTER, Madelyn | JR | Fontbonne | 12.68 | Culver Stockton Wildcat Open |
| 7 | MORRIS, Kori | FR | Purdue Northwest | 13.07 | |
| 8 | DELARUE, Gabby | JR | Missouri Baptist | 13.20 |