| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HALL-BAKER, KeYonn | JR | Fontbonne | 55.84 | Titan Open 2023 |
| 2 | LAWSON, Tristan | SR | Missouri Baptist | 53.00 | |
| 3 | HACKETT, Jonathon | FR | Southwestern CC | 52.45 | https://www.athletic.net/result/31ivZOnhlIX8K2Mh1/ |
| 4 | NEELY, Elias | FR | Missouri Baptist | 51.48 | |
| 5 | BATEMAN, Carson | FR | Purdue Northwest | 52.43 | 52.43 GVSU Big Meet (Saturday) |
| 6 | WILLS, Tyus | FR | Southwestern CC | 53.00 | |
| 7 | WASHINGTON, Anthony | SO | Harris-Stowe St. | 55.09 | AMC Championships |
| 8 | SAVAGE, Jordan | SO | Harris-Stowe St. | 56.00 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | AMSTUTZ, Carson | FR | Saint Louis | 50.73 | |
| 2 | SIMMONS, Alex | FR | Purdue Northwest | 50.62 | |
| 3 | OLSON, Ben | FR | Southwestern CC | 50.00 | |
| 4 | HOPKINS, Danny | JR | Saint Louis | 49.28 | 2023 Boston University David Hemery Valentine Invite |
| 5 | MATTHEWS, Corrion | JR | Purdue Northwest | 49.92 | 2021 Kip Janvrin Open |
| 6 | BUCHANAN, Octavius | JR | Purdue Northwest | 50.33 | 2022 Gulf South Conference Championships |
| 7 | NORTON, Robert | SO | Southwestern CC | 50.62 | 2022 Kip Janvrin Open |
| 8 | HINES, Jahari | JR | Health Sciences and Pharmacy | 51.13 | AMC Championships |