| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Oakville High School | A | Dawkins | Anderson | BLICK | WOEHL | NT | Unverified: |
| 3 | STEAM Academy at McCluer South-B | A | NT | Unverified: | ||||
| 4 | Normandy High School | A | NT | Unverified: | ||||
| 5 | Hazelwood Central High School | A | Anthony | Allen | Diagne | Nance | NT | Unverified: |
| 6 | Hazelwood West High School | A | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cardinal Ritter High School | A | NT | Unverified: | ||||
| 2 | Vashon High School | A | Aaron | Brown | Cody | Taylor | NT | Unverified: |
| 3 | Lift for Life Academy Charter Hi | A | Ridgel | Young | Hightower | Walker | NT | Unverified: |
| 4 | Belleville (East) | A | 1:47.00 | Unverified: | ||||
| 5 | East St. Louis (Sr.) | A | Coleman | Smith | Ivy | Jackson | 3:56.00 | Unverified: |
| 6 | Riverview Gardens High School | A | NT | Unverified: | ||||
| 7 | University City High School | A | Davis | Holland | Jennings | Nylon | NT | Unverified: |
| 8 | McCluer North High School | A | Moore | Webb | Carroll | Gordon | NT | Unverified: |