| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hazelwood Central High School | A | Miles | Griffin | White | Scruggs | NT | Unverified: |
| 2 | STEAM Academy at McCluer South-B | A | Simpson-Johnson | Taylor | Jimmerson | Shearer | NT | Unverified: |
| 3 | Vashon High School | A | Evans | Simpson-Bey | Brooks | Valentine | NT | Unverified: |
| 4 | University City High School | A | Elam | Smith | Simon | Henry | NT | Unverified: |
| 5 | Nerinx Hall High School | A | NT | Unverified: | ||||
| 6 | Lift for Life Academy Charter Hi | A | Taylor | Anthony | Worthem | Williams | NT | Unverified: |
| 7 | McCluer High School | A | Vasser | Robinson | Tate | Lewis | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cardinal Ritter High School | A | NT | Unverified: | ||||
| 2 | Hazelwood West High School | A | Taylor | Manning | Hayes | Edwards | NT | Unverified: |
| 3 | Ritenour High School | A | Bufford | Phillips | Shaw | Henderson | NT | Unverified: |
| 4 | Belleville (East) | A | Phalynn | Ronasia | Jacy | Ashayla | 1:54.00 | Unverified: |
| 5 | East St. Louis (Sr.) | A | 2:12.80 | Unverified: | ||||
| 6 | Riverview Gardens High School | A | NT | Unverified: | ||||
| 7 | Metro High School | A | Adams | McFarlane | Owens | Lewis | NT | Unverified: |
| 8 | McCluer North High School | A | Eubanks | Clayton | Seck | Goforth | NT | Unverified: |