| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Lift for Life Academy Charter Hi | A | Rogers | Freemon | Walker | Hall | NT | Unverified: |
| 4 | McCluer North High School | A | Lloyd | Strong | Webb | Raggs | NT | Unverified: |
| 5 | Riverview Gardens High School | A | NT | Unverified: | ||||
| 6 | Cardinal Ritter High School | A | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Westminster Christian Academy | A | Lee | Lowry | Monga | Crooks | NT | Unverified: |
| 2 | Hazelwood West High School | A | NT | Unverified: | ||||
| 3 | Belleville (East) | A | 4:28.00 | Unverified: | ||||
| 4 | East St. Louis (Sr.) | A | Mosley | Coleman | Sanders | Jackson | 1:07.00 | Unverified: |
| 5 | Normandy High School | A | Barnes | Crawford | Graham | Lenore | 4:15.30 | Unverified: |
| 6 | STEAM Academy at McCluer South-B | A | Boyd | Holliman | Moonchild | Hines | NT | Unverified: |
| 7 | Metro High School | A | Long | Keoshkerian | Long | Murch | NT | Unverified: |
| 8 | Hazelwood Central High School | A | Bragg | Atkins | Webster | Godfrey | NT | Unverified: |