| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Cardinal Ritter High School | A | NT | Unverified: | ||||
| 4 | Riverview Gardens High School | A | NT | Unverified: | ||||
| 5 | Westminster Christian Academy | A | Wilson | Hager | Egbert | Payne | NT | Unverified: |
| 6 | Hazelwood Central High School | A | Johnson | Davis | White | Foster | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | STEAM Academy at McCluer South-B | A | Gibson | Jimmerson | Boyd | Smith | NT | Unverified: |
| 2 | Metro High School | A | Calloway | Kamara | Small | Malone | NT | Unverified: |
| 3 | Hazelwood East High School | A | Smith | Rankins | Perry | Bryant | 5:01.00 | Unverified: |
| 4 | East St. Louis (Sr.) | A | 4:25.60 | Unverified: | ||||
| 5 | Belleville (East) | A | Gabrielle | Annyca | London | bria | 4:50.00 | Unverified: |
| 6 | Nerinx Hall High School | A | Albillo | McConnell | Hennessey | Clyne | NT | Unverified: |
| 7 | McCluer High School | A | Tate | Robinson | Marshall | Lawrence | NT | Unverified: |
| 8 | Ritenour High School | A | NT | Unverified: |