| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Oakville High School | A | Berringer | Kordic | Lawler | Maddox | NT | Unverified: |
| 3 | Hazelwood West High School | A | NT | Unverified: | ||||
| 4 | Lift for Life Academy Charter Hi | A | Jones | Freemon | Walker | Hightower | NT | Unverified: |
| 5 | Metro High School | A | Estrada | Galburt- Ruiz | Keoshkerian | Long | NT | Unverified: |
| 6 | Westminster Christian Academy | A | Crooks | Monga | Edgar | Warren | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Belleville (East) | A | 3:52.00 | Unverified: | ||||
| 2 | Cardinal Ritter High School | A | 3:49.30 | Unverified: | ||||
| 3 | McCluer North High School | A | Henderson | Gray | Green | Milton | 3:47.13 | Verified |
| 4 | Hazelwood Central High School | A | Bright | Martin | Thornton | Anthony | 3:41.21 | Verified |
| 5 | East St. Louis (Sr.) | A | Baker | Smith | Coleman | Ivy | 3:42.00 | Unverified: |
| 6 | Riverview Gardens High School | A | 3:47.27 | Verified | ||||
| 7 | STEAM Academy at McCluer South-B | A | Moonchild | Smith | Johnson | Holliman | 3:50.56 | Verified |
| 8 | Normandy High School | A | Barnes | Crawford | Johnson | Lenore | 4:13.95 | Verified |