| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STACEY, Jonathan | FR | McCluer High School | NT | Unverified: |
| 2 | HOLLAND, Jamal | FR | University City High School | NT | Unverified: |
| 3 | CROOKS, Demetri | FR | Westminster Christian Academy | NT | Unverified: |
| 4 | OETTING, Carter | FR | Oakville High School | NT | Unverified: |
| 5 | WILLIAMS, Jeremiah | FR | North County Christian | NT | Unverified: |
| 6 | PENNINGROTH, Nathan | FR | Hazelwood West High School | NT | Unverified: |
| 7 | BIHR, William | FR | Metro High School | NT | Unverified: |
| 8 | SYLLA, Mohamed | SO | Hazelwood West High School | NT | Unverified: |
| 9 | WRIGHT, Armani | SO | McCluer High School | NT | Unverified: |
| 10 | HILL, Antonio | FR | McCluer North High School | NT | Unverified: |
| 11 | BARNES, Jabori | FR | Normandy High School | NT | Unverified: |
| 12 | WYNNE, Jalen | SO | McCluer High School | NT | Unverified: |
| 13 | COTTLE, Ryan | FR | Hazelwood Central High School | NT | Unverified: |
| 14 | GALBURT- RUIZ, Martin | FR | Metro High School | NT | Unverified: |
| 15 | MASSEY, Chase | FR | North County Christian | NT | Unverified: |
| 16 | SIMMONS, Kornell | SO | Riverview Gardens High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | IVY, Darius | SO | East St. Louis (Sr.) | 2:08.26 | Verified |
| 2 | PERKINS, James | FR | Belleville (East) | 2:12.00 | Unverified: |
| 3 | DEBOIS, Marcus | FR | East St. Louis (Sr.) | 2:20.00 | Unverified: |
| 4 | CRAWFORD, Demetrius | SO | Normandy High School | 2:31.26 | Verified |
| 5 | MWANGI, Frank | FR | Hazelwood West High School | 2:32.15 | Verified |
| 6 | HUNTER, Arius | FR | Hazelwood Central High School | 2:44.73 | Verified |
| 7 | UNION JR., Charles | FR | Cardinal Ritter High School | 2:45.22 | Unverified: |
| 8 | KEOSHKERIAN, Benjamin | SO | Metro High School | 2:45.84 | Verified |
| 9 | BLANCHARD, Robert | SO | Hazelwood Central High School | 2:48.28 | Verified |
| 10 | HICKS, Christian | SO | Belleville (East) | 2:50.00 | Unverified: |
| 11 | JOHNSON, Soloman | FR | Normandy High School | 2:51.39 | Verified |
| 12 | EDGAR, Tate | FR | Westminster Christian Academy | NT | Unverified: |
| 13 | WILLIAMS, Jocques | FR | McCluer North High School | NT | Unverified: |
| 14 | LAWLER, Andrew | FR | Oakville High School | NT | Unverified: |
| 15 | HALL, London | FR | Lift for Life Academy Charter Hi | NT | Unverified: |
| 16 | AL-KENANI, Ali | FR | Oakville High School | NT | Unverified: |