| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BURSE, Gabrielle | FR | Hazelwood Central High School | NT | Unverified: |
| 2 | MORENO RUIZ, Julissa | FR | Ritenour High School | NT | Unverified: |
| 3 | SMITH, Trinity | SO | STEAM Academy at McCluer South-B | NT | Unverified: |
| 4 | DURNIN, Jaycee | FR | Ritenour High School | NT | Unverified: |
| 5 | HAWKINS, Dalyah | SO | East St. Louis (Sr.) | NT | Unverified: |
| 6 | LUETKEMEYER, Lucy | FR | Metro High School | NT | Unverified: |
| 7 | GIBSON, Haylee | SO | STEAM Academy at McCluer South-B | NT | Unverified: |
| 8 | CARTER, Caleigh | FR | North County Christian | NT | Unverified: |
| 9 | FENTON, Amiyah | FR | East St. Louis (Sr.) | NT | Unverified: |
| 10 | SIMMONS, Geriyah | FR | East St. Louis (Sr.) | NT | Unverified: |
| 11 | WINFIELD, Aaliyah | FR | Normandy High School | NT | Unverified: |
| 12 | ERHARD, Rowan | FR | Metro High School | NT | Unverified: |
| 13 | VALENTINE, Leela | FR | Vashon High School | NT | Unverified: |
| 14 | HARDY, Ariel | FR | Hazelwood Central High School | NT | Unverified: |
| 15 | WIGGLEY, Syllena | SO | McCluer North High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BRIA, johnson | SO | Belleville (East) | 2:40.00 | Verified |
| 2 | KENNEUBREW, Jaelyn | SO | Cardinal Ritter High School | 2:45.31 | Verified |
| 3 | ANNYCA, Wilson | SO | Belleville (East) | 2:50.00 | Unverified: |
| 4 | RODGERS, Aaliyah | SO | Incarnate Word Academy | 2:54.96 | Verified |
| 5 | KITCHENS, Kacy | FR | Hazelwood East High School | 2:57.68 | Verified |
| 6 | JONES, Aryanna | FR | Hazelwood Central High School | 3:19.19 | Verified |
| 7 | FAIR, D'Arie | SO | Hazelwood East High School | 3:21.64 | Verified |
| 8 | RANKINS, Diamond | FR | Hazelwood East High School | 3:27.00 | Unverified: |
| 9 | JACKSON, Kennedy | FR | Cardinal Ritter High School | NT | Unverified: |
| 10 | JENNERJOHN, Sarah | FR | Hazelwood West High School | NT | Unverified: |
| 12 | LAWRENCE, Azeria | FR | McCluer High School | NT | Unverified: |
| 14 | SMITH, Julian | FR | Ritenour High School | NT | Unverified: |
| 15 | BRYANT, Neveah | SO | Cardinal Ritter High School | NT | Unverified: |
| 16 | MARSHALL, Makara | JR | McCluer High School | NT | Unverified: |