| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Riverview Gardens High School | A | NT | Unverified: | ||||
| 3 | Vashon High School | A | Boyce | Brooks | Evans | Simpson-Bey | NT | Verified |
| 4 | Cardinal Ritter High School | A | 54.33 | Unverified: | ||||
| 5 | STEAM Academy at McCluer South-B | A | Boyd | Cromer | Johnson-Burns | Sheriff | 56.83 | Verified |
| 6 | Metro High School | A | Adams | Brinkley | Owens | Couilloud | NT | Unverified: |
| 7 | McCluer High School | A | Robinson | Lewis | Robinson | Vasser | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hazelwood West High School | A | Manning | Taylor | Thompson | Jackson | 52.76 | Verified |
| 2 | East St. Louis (Sr.) | A | 52.19 | Unverified: | ||||
| 3 | Hazelwood Central High School | A | Swanson | Witherspoon | Miles | Reed | NT | Unverified: |
| 4 | Belleville (East) | A | Zariah | Bacon | Tahjae | Kamryn | NT | Unverified: |
| 5 | Incarnate Word Academy | A | Crump | Allen | White | Tobias | 49.11 | Verified |
| 6 | McCluer North High School | A | Hammond | Clayton | Fenton | Lamb | 51.65 | Verified |
| 7 | Hazelwood East High School | A | Carter | Fitch | Glanton | K'Niyah | NT | Verified |
| 8 | Ritenour High School | A | Royal | Bufford | Bell | Jones | 53.83 | Verified |