| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Belleville (East) | A | 2:24.70 | Unverified: | ||||
| 4 | Hazelwood Central High School | A | George | Burns | Allen | Gunn | 1:36.89 | Unverified: Mike Rodgers Invitational |
| 5 | East St. Louis (Sr.) | A | Penn | Jackson | Harmon | Reed | 1:40.00 | Unverified: |
| 6 | Westminster Christian Academy | A | Lee | Skaggs | Warren | Lowry | NT | Unverified: |