| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | McCluer High School | A | Mitchell | Jones | Moore | Morgan | NT | Unverified: |
| 3 | Westminster Christian Academy | A | Brown | Hager | Wilson | Groat | NT | Unverified: |
| 4 | Vashon High School | A | Conner | Evans | Jones | Smith | 2:03.48 | Verified |
| 5 | Lift for Life Academy Charter Hi | A | Anthony | Worthem | Williams | Taylor | NT | Unverified: |
| 6 | Normandy High School | A | Bankhead | Phipps | Quilling | Winfield | NT | Unverified: |
| 7 | Incarnate Word Academy | A | Harris | Smart | Sanderson | Jones | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hazelwood West High School | A | Jackson | Manning | Taylor | Hayes | 1:59.59 | Verified |
| 2 | Ritenour High School | A | Bell | Green-williams | Royal | 1:57.88 | Verified | |
| 3 | Riverview Gardens High School | A | 1:52.35 | Unverified: | ||||
| 4 | Belleville (East) | A | Tahjae | Darielle | London | KaLiyah | 1:50.00 | Unverified: |
| 5 | Cardinal Ritter High School | A | 1:50.77 | Unverified: | ||||
| 6 | East St. Louis (Sr.) | A | 1:52.75 | Unverified: | ||||
| 7 | STEAM Academy at McCluer South-B | A | Boyd | Cromer | Gibson | Jimmerson | 1:58.22 | Unverified: |
| 8 | Hazelwood Central High School | A | McCanery | Davis | Johnson | Mayes | 1:59.93 | Unverified: |