| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Riverview Gardens High School | A | Davis | Harris-Grayer | Davis | Coleman | NT | Unverified: |
| 2 | Vashon High School | A | Evans | Jones | Conner | Simpson-Bey | NT | Verified |
| 3 | STEAM Academy at McCluer South-B | A | Hubbard | Bailey | Young | Taylor | 1:59.22 | Unverified: |
| 4 | McCluer North High School | A | Clayton | Hart | Moore | Davis | 1:54.19 | Verified |
| 5 | Hazelwood Central High School | A | Griffin | Wicks | Scruggs | Stamps | 1:56.93 | Unverified: |
| 6 | East St. Louis (Sr.) | A | 2:00.54 | Unverified: | ||||
| 7 | Ritenour High School | A | Phillips | Henderson | Shaw | Monroe | NT | Unverified: |