| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LYSON, Mikayla | FR | Rock Bridge High School | NT | Unverified: |
| 2 | BAKER, Lucy | SO | Christian Fellowship School | NT | Unverified: |
| 3 | NEWBY, Serena | SR | Battle High School | 54.06 | Verified |
| 4 | BUTLER, Jordyn | SR | Battle High School | 50.60 | Verified |
| 5 | CARTER, Ciara | JR | Battle High School | 53.63 | Verified |
| 6 | NUSBAUM, Eden | SO | Hickman High School | 54.26 | Verified |
| 7 | PARSONS, Grace | SR | Rock Bridge High School | NT | Unverified: |