| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MOHRLAND, Caleb | FR | Christian Fellowship School | NT | Unverified: |
| 2 | KERN, David | FR | Rock Bridge High School | NT | Unverified: |
| 3 | ROONEY, John | SO | Rock Bridge High School | NT | Unverified: |
| 4 | METTU, Rithvik | FR | Rock Bridge High School | NT | Unverified: |
| 5 | WALLER, Joseph | FR | Hickman High School | NT | Unverified: |
| 6 | RIBIAT, Phoenix | SO | Hickman High School | NT | Unverified: |
| 7 | MASCHMANN, Benjamin | FR | Rock Bridge High School | NT | Unverified: |
| 8 | CANLAS, Noah | FR | Rock Bridge High School | NT | Unverified: |
| 9 | NOVAK, Elijah | FR | Rock Bridge High School | NT | Unverified: |
| 10 | BUCHLY, Killian | FR | Rock Bridge High School | NT | Unverified: |
| 11 | SIMMONS, Zev | FR | Rock Bridge High School | NT | Unverified: |
| 12 | BUXTON, Coen | SO | Battle High School | NT | Unverified: |
| 13 | DETTMAR, David | 8 | Rock Bridge High School | NT | Unverified: |
| 14 | REID, Tyler | FR | Rock Bridge High School | NT | Unverified: |
| 15 | PATEL, Vedant | FR | Rock Bridge High School | NT | Unverified: |
| 16 | BEDFORD, Mason | FR | Battle High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NEIL, Tanner | JR | Rock Bridge High School | 4:54.80 | Verified |
| 2 | MUHLENBRUCK, Elliot | SR | Hickman High School | 4:54.99 | Verified |
| 3 | HUDSON, Cook | FR | Rock Bridge High School | 4:58.00 | Unverified: |
| 4 | LEMBKE, Carter | FR | Rock Bridge High School | 5:01.08 | Verified |
| 5 | HULEN, Colton | FR | Rock Bridge High School | 5:02.00 | Unverified: |
| 6 | HALL, Mikel | FR | Battle High School | 5:13.43 | Verified |
| 7 | TICGELAOR, Ben | JR | Battle High School | 5:15.83 | Verified |
| 8 | ALMASRI, Hamzah | FR | Rock Bridge High School | 5:20.00 | Unverified: |
| 9 | IMBODEN, Johnny | SR | Rock Bridge High School | 5:21.32 | Verified |
| 10 | TIEMEYER, Jackson | SO | Rock Bridge High School | 5:24.03 | Verified |
| 11 | BREDEHOEFT, Noah | SR | Rock Bridge High School | 5:25.00 | Unverified: |
| 12 | MOHRLAND, David | JR | Rock Bridge High School | 5:32.85 | Verified |
| 13 | MCGOWAN, Kyle | SO | Christian Fellowship School | 5:42.48 | Verified |
| 14 | TICGELAOR, Alex | JR | Battle High School | 5:48.45 | Verified |
| 15 | KAUFFMAN, Nathaniel | SO | Christian Fellowship School | 5:48.56 | Verified |
| 16 | SILL, Noah | SO | Battle High School | 5:50.44 | Verified |