| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | RAMADAM, Layaly | FR | Lindenwood | 1:08.13 | Blue-White Classic |
| 4 | WITTIG, Savannah | FR | Wis.-Parkside | 1:05.34 | Lake Michigan Open |
| 5 | EDWARDS, Karrington | FR | Murray State | 1:06.21 | Margaret Simmons Invite 23 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | PETERSON, Amber | FR | Lindenwood | 1:04.01 | |
| 3 | ARIAS, Alysia | FR | Lewis | 1:02.20 | Miner Invitational |
| 4 | CLARK, Olivia | JR | Southern Indiana | 1:01.00 | |
| 5 | BROWN, Janay | JR | Wis.-Parkside | 1:02.00 | |
| 6 | GORDON, Daja | SR | SE Missouri | 1:03.36 | 2023 South Alabama Invitational |
| 7 | HUMPHRY, Zarra | SR | Murray State | 1:04.26 | 2022 Kentucky Track & Field Invitational |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BLADES, Reneé | FR | Tennessee-Martin | 1:00.44 | PNC Bank Bellarmine Classic |
| 2 | CHAPMAN, Janay | JR | Tennessee-Martin | 59.34 | Rhodes College 7-Way Meet |
| 3 | DWAAH, Leanza | JR | SE Missouri | 57.98 | 2023 South Alabama Invitational |
| 4 | LEWIS, Melody | FR | Memphis | 57.75 | |
| 5 | CHALMERS, Kyla | SR | Lewis | 57.80 | GLVC Track and Field Championships |
| 6 | GORDON, Alaina | FR | Tennessee-Martin | 59.00 | |
| 7 | WYATT, JayleAna | SO | Tennessee-Martin | 59.84 | ASU Red Wolf Open |
| 8 | SMITH, Sanaa | SO | Tennessee-Martin | 1:00.52 | OVC Outdoor Track & Field Championships |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ANDERSON, Hanna | SR | SIU Edwardsville | 57.31 | ASUN Outdoor Track & Field Championships |
| 2 | RUSSINA, Gabby | JR | Lindenwood | 56.68 | OVC championship |
| 3 | MILES, Breanna | JR | SE Missouri | 55.82 | OVC championship |
| 4 | HERRERO-YANEZ, Lucia | JR | Murray State | 55.42 | OVC championship |
| 5 | ATLAS, Tylantiss | SR | Murray State | 55.44 | 2021 GNAC Outdoor Championships |
| 6 | HARDIN, Laila | JR | SE Missouri | 56.58 | OVC championship |
| 7 | CARTER, Kailah | JR | SIU Edwardsville | 57.06 | OVC championship |
| 8 | GREY, Vashti | SO | Southern Illinois | 57.47 | USF Bulls Invitational |