| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ALFORD, Clara | FR | Tennessee-Martin | 5:20.00 | |
| 2 | BARRETT, Gillian | FR | Memphis | 5:20.72 | Ole Miss Classic 2023 |
| 3 | PROVOST, Parker | FR | Southern Indiana | 5:24.62 | Jim Vargo Invitational |
| 4 | RODRIGUEZ, Carmen | SR | Southern Indiana | 5:33.23 | Brescia Bearcat Classic |
| 5 | QUEVEDO, Cecilia | JR | Wis.-Parkside | 5:38.45 | Cal State LA Twilight (College/Univ) |
| 6 | ANTHONIES, Courtney | FR | SIU Edwardsville | NT | |
| 7 | BROWN, Gabrielle | FR | Tennessee-Martin | NT | |
| 8 | POPE, Sophey | FR | Arkansas Tech | NT | |
| 9 | ZINKE, Colleen | FR | McKendree | NT | |
| 10 | MANLEY, Allison | FR | Tennessee-Martin | NT | |
| 11 | PASCHAL, Sophia | FR | McKendree | NT | |
| 12 | TIEFFEL, Peyton | FR | SIU Edwardsville | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | AMAYA, Audrey | FR | Wis.-Parkside | 5:00.00 | |
| 2 | DAY, Breanna | JR | Murray State | 5:00.55 | 2021 Kentucky Invtational |
| 3 | ANDERSON, Ashleigh | FR | Memphis | 5:00.66 | Ole Miss Classic 2023 |
| 4 | STANLEY, Avery | FR | Arkansas Tech | 5:03.51 | Richard Martin Invitational |
| 5 | SPARR, Alayna | FR | SE Missouri | 5:04.23 | |
| 6 | THOMPSON, Emma | SO | Southern Indiana | 5:05.19 | Jim Vargo Invitational |
| 7 | WEST, Felicity | JR | Arkansas Tech | 5:06.63 | 2021 Great American Conference Track & Field Championships |
| 8 | LIVINGSTON, Shelby | JR | Southern Indiana | 5:07.00 | |
| 9 | WOOD, Allison | FR | Murray State | 5:10.00 | |
| 10 | HOST, Jocelyn | FR | Murray State | 5:10.00 | |
| 11 | HOGER, Hallie | FR | Wis.-Parkside | 5:10.00 | |
| 12 | WARE, Hope | FR | Murray State | 5:16.18 | Margaret Simmons Invite 23 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MELLOR, Erika | SO | SE Missouri | 4:20.00 | Pacer |
| 2 | WEGMANN, Katie | SR | SE Missouri | 4:27.15 | 2023 Mississippi State Bulldog Relays |
| 3 | FOX, Taylor | JR | SE Missouri | 4:29.73 | 2022 Indiana Invitational |
| 4 | COMASTRI, Audrey | JR | Southern Indiana | 4:33.00 | |
| 5 | REMPE, Emily | FR | Southern Indiana | 4:33.00 | |
| 6 | MORPHEW, Allison | JR | Southern Indiana | 4:40.92 | Bellarmine Jim Vargo Invitational |
| 7 | PAYTON, Brianna | JR | SE Missouri | 4:46.01 | OVC Outdoor Track & Field Championships |
| 8 | MITROVIC, Sonja | JR | Wis.-Parkside | 4:50.00 | |
| 9 | EKE, Reezon | SO | Memphis | 4:50.23 | Ole Miss Classic 2023 |
| 10 | ANTHONIES, Stephanie | JR | Southern Illinois | 4:52.92 | Vanderbilt Black & Gold Invitational |
| 11 | ROY, Maggie | FR | Tennessee-Martin | 4:55.32 | Margaret Simmons Invite 23 |
| 12 | FLIEGE, Layla | FR | SE Missouri | 4:55.72 | 2023 Mississippi State Bulldog Relays |
| 13 | SMITH, Ainsley | SO | Murray State | 4:59.00 | |
| 14 | KURFMAN, Lydia | SO | Memphis | 4:59.49 | Memphis Tiger Invitational |