| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Murray State | B | Muller | Huber | Marshall | Shaffer | 49.50 | |
| 2 | SIU Edwardsville | A | Williams | Oladipupo | Allen | Reynolds | 48.68 | OVC Outdoor Track & Field Championships |
| 3 | SE Missouri | A | Williams | Miles | Dwaah | Gordon | 46.30 | OVC championship |
| 4 | Murray State | A | Bostick | James | Herrero-Yanez | Hagans | 45.14 | Vanderbilt Black & Gold Invitational |
| 5 | Lewis | A | Martindale | Ogbuli | Wyman | Hernandez | 46.20 | |
| 6 | Tennessee-Martin | A | Taylor | Gordon | Wyatt | Chapman | 47.60 | Margaret Simmons Invite 23 |
| 7 | SIU Edwardsville | B | Smith | Sullivan | Jones | Bivins | 48.68 | OVC Outdoor Track & Field Championships |