| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Murray State | D | Muller | Shaffer | Thomas | Ware | 4:08.00 | |
| 4 | Murray State | C | Smith | Ware | Humphry | Huber | 4:05.00 | |
| 5 | Lindenwood | A | Hoyte | Hubbell | Peterson | Ramadan | 4:06.92 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Southern Indiana | A | Clark | Brown | Rempe | Comastri | 4:03.00 | |
| 2 | Tennessee-Martin | B | Wyatt | Blades | Smith | Jackson | 3:59.37 | OVC Outdoor Track & Field Championships |
| 3 | SE Missouri | A | Gray | Fox | Payton | Mellor | 3:57.34 | |
| 4 | Murray State | A | Atlas | Herrero-Yanez | Hagans | Bostick | 3:41.71 | Outdoor Music City Challenge |
| 5 | SIU Edwardsville | A | Allen | Anderson | Horton | Carter | 3:56.18 | OVC championship |
| 6 | Murray State | B | Joseph | James | Bostick | Davis | 3:58.00 | |
| 7 | Tennessee-Martin | A | Gordon | Eimer | Chapman | Roy | 3:59.37 | OVC Outdoor Track & Field Championships |
| 8 | McKendree | A | King | Paschal | Douglas | Lucas | 4:03.80 | GLVC Outdoor Championships |