| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BUMPERS, Johnathan | SR | McKendree | NT | |
| 2 | GAITERS, James | FR | Southern Illinois | NT | |
| 3 | BELCHER, Caden | FR | SIU Edwardsville | 59.15 | 2023 EIU Big Blue |
| 4 | OXFORD, Landon | FR | Lindenwood | 57.79 | 2023 EIU Big Blue |
| 5 | JUSZCZYK, Julian | FR | McKendree | 58.71 | |
| 6 | LEHMAN, Taylor | SO | SIU Edwardsville | 59.84 | 2023 EIU Big Blue |
| 7 | BREMA, Momoh | SR | SE Missouri | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FRY, Brady | SR | Tennessee-Martin | 56.22 | OVC Outdoor Track & Field Championships |
| 2 | SIMPSON, Jacari | SO | Memphis | 55.99 | Rhodes College Track & Field Invitational |
| 3 | ASHTON, Shane | FR | Southern Illinois | 54.33 | USF Bulls Invitational |
| 4 | WOOD, Brock | JR | SE Missouri | 53.22 | OVC championship |
| 5 | SONNTAG, Tommy | JR | Lindenwood | 53.38 | GLVC Track and Field Championships |
| 6 | HOLMES, Brian | FR | Lewis | 55.30 | Miner Invitational |
| 7 | KNAPP, Cole | JR | SIU Edwardsville | 56.22 | OVC championship |
| 8 | KILLMER, Aidan | FR | Lewis | 57.50 |