| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CLUVER, Maluke | Unattached | 2:00.00 | ||
| 2 | BUECHLEIN, Elliott | FR | Southern Indiana | 2:00.00 | |
| 3 | BLASSINGAME, Jacob | FR | SE Missouri | 2:01.00 | |
| 4 | EFTINK, Clayton | SO | SE Missouri | 2:01.47 | 2021 Joey Haines Invite |
| 5 | MOSTAFFA, Jose | SO | Lindenwood | 2:01.68 | |
| 6 | ELLIS, Ross | SR | Southern Indiana | 2:03.00 | |
| 7 | DAVIS, Jackson | FR | Southern Indiana | 2:04.00 | |
| 8 | MAXWELL, Timothy | JR | Lindenwood | 2:04.11 | |
| 9 | BYRNE, Declan | FR | Memphis | 2:07.88 | Ole Miss Classic 2023 |
| 10 | CASE, Matt | SO | Lindenwood | 2:17.03 | 2023 Gladstein Invitational |
| 11 | COUPLAND, Sam | FR | Lindenwood | 2:18.46 | 2023 EIU Big Blue |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PRIMEAU, Louis | FR | SE Missouri | 1:56.17 | |
| 2 | ZIMMERMAN, Tyler | FR | Southern Indiana | 1:56.50 | |
| 3 | DEPUYDT, Austin | SO | Wis.-Parkside | 1:56.58 | Warhawk Invitational & Multi |
| 4 | GARRETT, Tyler | JR | Southern Indiana | 1:56.83 | GLVC Track and Field Championships |
| 5 | PFLANZ, Rick | SO | Southern Indiana | 1:56.90 | |
| 6 | GILO, Wimachmorr | SO | Southern Indiana | 1:57.50 | |
| 7 | CABALTERA, Michael | SO | Wis.-Parkside | 1:57.71 | Parkside Classic |
| 8 | HEINEMANN, Luke | SO | Southern Indiana | 1:57.86 | OVC championship |
| 9 | JOHNSON, Erik | JR | Wis.-Parkside | 1:58.30 | Parkside Classic |
| 10 | AKEY, Konner | SO | Wis.-Parkside | 1:59.02 | Minnesota State Massive Meet #2 |
| 11 | BUSH, John | FR | McKendree | 1:59.36 | 2023 EIU Friday Night Special |
| 12 | CULLINS, Emmanuel | SR | Memphis | 1:59.39 | War Eagle Invitational 2022 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GARRETT, Terrico | JR | SE Missouri | 1:49.44 | Tennessee Relays |
| 2 | WEAVER, Michael | SR | SE Missouri | 1:50.00 | Pacer |
| 3 | TOURON, Maxime | SO | Lindenwood | 1:50.53 | Missouri S&T Qualifier |
| 4 | WALDVOGEL, Sean | SO | Wis.-Parkside | 1:52.65 | GVSU Bill Clinger Classic |
| 5 | FORD, Dan | SR | Lewis | 1:52.94 | GLVC Track and Field Championships |
| 6 | SPLITT, Justin | JR | SE Missouri | 1:53.13 | OVC Outdoor Track & Field Championships |
| 7 | CRAWFORD, Mark | JR | SE Missouri | 1:54.02 | OVC Outdoor Track & Field Championships |
| 8 | FOOTE, Hawken | JR | Tennessee-Martin | 1:54.03 | OVC Indoor Track & Field Championships |
| 9 | HUDSON, Brody | SO | Lindenwood | 1:54.11 | |
| 10 | POTTSCHMIDT, Dillon | SO | Lewis | 1:54.33 | 200 track conversion |
| 11 | SMITH, Aubrey | SO | Lewis | 1:54.43 | GLVC Track and Field Championships |
| 12 | HESS, Cole | FR | Southern Indiana | 1:56.00 |