| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BOST, Felicity | SO | Memphis | 2:29.89 | Music City Challenge |
| 2 | HOGER, Hallie | FR | Wis.-Parkside | 2:31.56 | Firebird First Invitational |
| 3 | QUEVEDO, Cecilia | JR | Wis.-Parkside | 2:32.98 | Parkside Classic |
| 4 | HOST, Jocelyn | FR | Murray State | 2:33.00 | |
| 5 | WOOD, Allison | FR | Murray State | 2:33.00 | |
| 6 | WARE, Hope | FR | Murray State | 2:34.07 | Bellarmine Open |
| 7 | DAY, Breanna | JR | Murray State | 2:36.00 | |
| 8 | CUSTER, Akira | JR | Southern Illinois | NT | |
| 9 | SMITH, Ainsley | SO | Murray State | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FLIEGE, Layla | FR | SE Missouri | 2:19.67 | |
| 2 | EKE, Reezon | SO | Memphis | 2:20.33 | Crimson Tide Invitational |
| 3 | ANDERSON, Ashleigh | FR | Memphis | 2:20.38 | Ole Miss Classic 2023 |
| 4 | HUBBELL, Emma | JR | Lindenwood | 2:21.33 | |
| 5 | AMAYA, Audrey | FR | Wis.-Parkside | 2:23.00 | |
| 6 | LUCAS, Reagan | SO | McKendree | 2:25.68 | Miner Invitational |
| 7 | SPARR, Alayna | FR | SE Missouri | 2:26.00 | |
| 8 | TEPE, Madelyn | SR | Lewis | 2:26.00 | |
| 9 | RAMADAN, Jenna | FR | Lindenwood | 2:29.44 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MELLOR, Erika | SO | SE Missouri | 2:10.00 | Pacer |
| 2 | BROWN, Emma | SR | Southern Indiana | 2:13.59 | 2022 Joey Haines Invite |
| 3 | GRAY, Madalyn | SO | SE Missouri | 2:14.22 | PNC Bank Bellarmine Classic |
| 4 | PAYTON, Brianna | JR | SE Missouri | 2:15.42 | OVC Outdoor Track & Field Championships |
| 5 | HOYTE, Rosette | SR | Lindenwood | 2:17.03 | GLVC Track and Field Championships |
| 6 | EIMER, Rita | SO | Tennessee-Martin | 2:18.36 | PNC Bank Bellarmine Classic |
| 7 | ROY, Maggie | FR | Tennessee-Martin | 2:18.42 | Margaret Simmons Invite 23 |
| 8 | MITROVIC, Sonja | JR | Wis.-Parkside | 2:18.55 | GLIAC Indoor Track & Field Championships |
| 9 | HORTON, Gabrielle | JR | SIU Edwardsville | 2:18.74 | OVC championship |