| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Riverview Gardens High School | A | Smith | Smith | Robinson | Gladden | NT | Unverified: |
| 2 | Hazelwood West High School | A | Jones | Squires | Fleming | Abdul-Mumin | 4:46.94 | Verified |
| 3 | STEAM Academy at McCluer South-B | A | Green | Saunders | Cromer | Rufus-Smith | 4:28.00 | Unverified: |
| 4 | Hazelwood East High School | A | Fitch | Carter | Glanton | Buchannan | 4:36.60 | Verified |
| 5 | Hazelwood Central High School | A | Witherspoon | Elam | Simmons | Wilkins | NT | Unverified: |