| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Timberland High School | A | Volkman | Neubauer | Engemann | Jasper | NT | Unverified: |
| 3 | Washington High School | A | Smith | Stegen | Riley | Henderson | 1:50.00 | Unverified: |
| 4 | Francis Howell High School | A | 1:35.61 | Verified | ||||
| 5 | Christian Brothers College High | A | Jones | Stimage | Washington | Jackson | 1:37.00 | Unverified: |
| 6 | Francis Howell Central High Scho | A | Bell | Gil Farias | Payton | Thornton | NT | Unverified: |
| 7 | Troy Buchanan High School | A | NT | Unverified: |