| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Francis Howell Central High Scho | A | Gil Farias | Jordan | Cole | Stock | DNS | Unverified: |
| 3 | Christian Brothers College High | A | Rodgers | Washington | Hughes | Leicht | 4:04.80 | Verified |
| 4 | Francis Howell High School | A | 3:32.59 | Verified | ||||
| 5 | Washington High School | A | Riley | Stegen | Wieland | 4:00.00 | Unverified: | |
| 6 | Timberland High School | A | Winston | Barnes | Litchfield | Katambwa | NT | Unverified: |
| 7 | Troy Buchanan High School | A | DNS |