| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DIAZ, Courtney | SR | Steelville High School | 5:47.31 | Verified |
| 2 | FULLIAM, Kaylee | JR | Cuba High School | 5:51.06 | Verified |
| 3 | TURNBOUGH, Audrey | JR | Steelville High School | 5:58.25 | Verified |
| 4 | CAQUELIN, Akaela | SO | Newburg High School | 5:59.34 | Verified |
| 5 | GEHLERT, Hannah | FR | Owensville High School | 6:22.87 | Verified |
| 6 | FITE, Brooklyn | FR | Newburg High School | 6:26.17 | Verified |
| 7 | GIBSON, Abbie | SO | Owensville High School | 6:33.45 | Verified |
| 8 | BASHAM, Cadynce | FR | Cuba High School | 6:53.43 | Verified |
| 9 | HARDCASTLE, Patricia | FR | Cuba High School | 7:08.76 | Verified |
| 10 | COLLINSON, Lydia | FR | Steelville High School | NT | Unverified: |