| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CAQUELIN, Akaela | SO | Newburg High School | 2:42.78 | Verified |
| 2 | TURNBOUGH, Audrey | JR | Steelville High School | 2:43.30 | Verified |
| 3 | IJAMES, Izzabelle | JR | Cuba High School | 2:47.79 | Verified |
| 4 | FITE, Brooklyn | FR | Newburg High School | 2:53.80 | Verified |
| 5 | GEHLERT, Hannah | FR | Owensville High School | 2:55.41 | Verified |
| 6 | BREEDLOVE, Jenna | FR | Owensville High School | 2:58.56 | Verified |
| 7 | WEINLAND, Peyton | JR | Owensville High School | 3:03.52 | Verified |
| 8 | GIBSON, Abbie | SO | Owensville High School | 3:03.83 | Verified |
| 9 | WOOLS, Kailey | SO | Cuba High School | 3:05.59 | Verified |
| 10 | GEBHART, Mia | JR | Bourbon High School | 3:12.12 | Verified |
| 11 | PRATT, Ciarra | JR | Newburg High School | 3:19.01 | Verified |
| 12 | STOKER, Hannah | JR | Owensville High School | 3:23.65 | Verified |
| 13 | COLLINSON, Lydia | FR | Steelville High School | NT | Unverified: |
| 14 | PRYOR, Elivia | FR | Steelville High School | NT | Unverified: |