| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BROOKS, Peyton | FR | Steelville High School | NT | Unverified: |
| 2 | EVANS, Norah | JR | Steelville High School | 1:00.65 | Verified |
| 3 | BARTLE, Raylee | SO | Bourbon High School | 58.13 | Verified |
| 4 | TWYMAN, Corra | SR | Newburg High School | 50.31 | Verified |
| 5 | WIGGER, Natalie | SO | Viburnum High School | 53.42 | Verified |
| 6 | BLANKENSHIP, Merrick | SO | Owensville High School | 58.55 | Verified |
| 7 | SHARP, Theadora | JR | Cuba High School | 1:00.99 | Verified |
| 8 | WEHMEYER, Emma-lee | FR | Owensville High School | NT | Unverified: |