| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clayton High School | A | Stern | Sanders | Musiek | Ramirez Taylor | NT | Unverified: |
| 2 | Affton High School | A | Smith | Skinner | Cella | Wilson | NT | Unverified: |
| 3 | Mehlville High School | B | Bond | Hastings | Mathews | McGinnist | 2:14.96 | Unverified: Conference |
| 4 | Mehlville High School | A | Roth | TREVISANO | Nasufovic | Ramos | 1:59.92 | Unverified: Conference |
| 5 | Oakville High School | A | Czuppon | Mueller | JONAS | SQUIRES | 2:00.00 | Unverified: Oakville/Mehlville Dual |
| 6 | Clayton High School | B | Kabargina | Wolpert | Zaidman | Pittman | NT | Unverified: |
| 7 | Affton High School | B | McFee | Manuel | Olesia | Lang | NT | Unverified: |