| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Affton High School | B | NT | Unverified: | ||||
| 3 | Vianney High School | A | Turmelle | Lindsey | Miesner | Backer | NT | Unverified: |
| 4 | Jennings High School | A | Martin | Smith | McCoy | Wiley | 4:17.48 | Verified |
| 5 | Affton High School | A | Lovelace | Moehlenbrock | Pashkov | Morales | NT | Unverified: |
| 6 | Oakville High School | A | ALTENBERND | GOLD | LOUVIERRE | Lawler | NT | Unverified: |