| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Affton High School | A | Daughtry | Cousins | Smith | Olesia | NT | Unverified: |
| 3 | Mehlville High School | B | Brindley | Escamilla | Gutjahr | McClusky | 5:16.18 | Unverified: Conference |
| 4 | Oakville High School | A | Czuppon | JONAS | BROWER | SQUIRES | 4:40.00 | Unverified: Oakville/Mehlville Dual |
| 5 | Mehlville High School | A | O'Brien | Palmer | Ramos | Shores | 4:48.51 | Unverified: Conference |
| 6 | Affton High School | B | Hudson | Lang | McFee | Daniel | NT | Unverified: |