| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Wright City High School | A | NT | ||||
| 4 | Helias Catholic High School | A | Verslues | Harrison | Edwards | Struemph | 4:28.72 |
| 5 | Helias Catholic High School | B | Marke | Broker | Lock | French | 4:28.72 |
| 6 | Sullivan High School | A | Mayberry | McClain | Carpenter | Cabanlit | NT |