| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | HALL, Caleb | SO | Wright City High School | 54.82 |
| 2 | STONNER, Sam | FR | Helias Catholic High School | 51.32 |
| 3 | SCHMIDT, Julian | SO | Helias Catholic High School | 45.66 |
| 4 | MALMSTROM, Matthew | JR | Helias Catholic High School | 38.44 |
| 5 | HOELSCHER, Landon | SR | Helias Catholic High School | 45.04 |
| 6 | MCGOWAN, John | SO | Wright City High School | 49.44 |
| 7 | SUMMERS, Jayson | SO | Sullivan High School | 52.72 |
| 8 | RULO, Landon | FR | St. Clair High School | NT |